The hook effect or the prozone effect is a type of interference which plagues certain immunoassays and nephelometric assays, resulting in false negatives or . objetivos: conocer las diferentes técnicas de aglutinación usadas en el laboratorio. adquirir habilidad y destrezas en las técnicas de. Prozone effect: No prozone effect was detected upon mg/dL. 4. . Efecto prozona: No se observa efecto prozona hasta valores de

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Retrieved from ” https: Retrieved 3 February However, when the serum is diluted, the blocking antibody is as well and its concentration decreases enough for the proper precipitation evecto to occur.

By using this site, you agree to the Terms of Use and Privacy Policy. Because no agglutination occurs, the test is interpreted as negative. The hook effect or the prozone effect is a type of interference which plagues certain immunoassays and nephelometric assaysresulting in false negatives or inaccurately proona results.


In this case, free antigen is in competition with captured antigen for detection antibody binding. The serological test is mainly seen in the precipitation reaction.

Examples include high levels of syphilis antibodies in HIV patients or high levels of cryptococcal antigen leading to false negative tests in undiluted samples. The antibody that fails to react is known as the blocking antibody and prevents the precipitating antibody from binding to the antigens.

Thus the proper precipitation reaction does not take place.

Hook effect – Wikipedia

Views Read Edit View history. From Wikipedia, prozoa free encyclopedia. In this case, no sandwich can be formed by the capturing antibody, the antigen and the detection antibody. If the antibodies agglutinate with the antigen to form immune complexesthen the test is interpreted as positive.

Term Bank – efecto prozona – Spanish English Dictionary

The phenomenon is caused by very high concentrations of a particular analyte efcto antibody and is most prevalent in one-step sandwich immunoassays. Other common forms of interference include antibody interference, cross-reactivity and signal interference. In an agglutination test, a person’s serum which contains antibodies is added to a test tubewhich contains a particular antigen.


However, if too many antibodies are present that can bind to the antigen, then the antigenic sites are coated by antibodies, and few or no antibodies directed toward the pathogen are able to bind more than one antigenic particle. Has its time arrived?

This page was last edited on 14 Augustat The range of relatively high antibody concentrations within which no reaction occurs is called the prozone. The Clinical Biochemist Reviews. In this case, the result is a false negative.

The effect can also occur because of antigen excess, when both the capture and detection antibodies become saturated by the high analyte concentration.