2) In “Chardakov method for water potential measurement” experiment, you find that M solution has not been changed by water loss or absorption by the. Chardakov Technique. Plant Physiology. UNI. Incubating tissue in solution. Pre- incubation. Incubation. Post-incubation. Save for later measurement. Did solution change concentration? Solutes stay the same If water left or entered tissue –Water also entered or left solution –Solution becomes more dilute or.
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Then, the percent change in weight of the tissue is plotted versus solution concentration or osmotic potential. The water potential of the tissue is considered to be equal the osmotic potential of the incubating solution at which there is no change in tissue weight i. Examine the cores as chardakovv weigh them. Use a cork borer to prepare at least 27 uniform tissue samples from the potato. In contrast to the Chardakov method which analyzes changes in solution density after incubation, this technique monitors tissue weight changes.
Water potential values determined by this method may be slightly more negative than those obtained by the Chardokov method. Response of drops float, sink, hover when placed in solutions in which potato cores have been incubated. Which is more accurate? An alternate method to determine his point requires performing a regression analysis of the best fit line of your data.
Mix the tubes thoroughly with a vortex mixer. Hypertonic Concentration with higher solute concentration and less water concentration Hypotonic lower solute concentration and more water concentration. Registration Forgot your password? Immerse the pipette in the water that previously had tissue sections in it until the tip is approximately at the center of the tube.
Be sure not to include any fragments of the skin. Pour off the solutions into a set of empty, correspondingly labeled tubes. Cut them to the same length with a razor blade ca. What is the water potential calculated by this method?
Published by Sabrina Grant Modified over 2 years ago. Work quickly to minimize evaporation and keep the tissue wrapped in a moist towel. My presentations Profile Feedback Log out. The solution gains or looses water depending on the water potential of the tissue.
Chardakov method Essay – Free Papers and Essays Examples
Draw the best fit line for your data. If the density of a solution does not change no net movement of water then this solution has the same water potential as the tissues that were incubated in it. Osmosis diffusion methox water through a selectively permeable membrane Membrane X is permeable to water but not to protein Which side has the highest concentration.
Incubate the cores for at least 1. Cytology Osmosis and Water relations. Incubate the cores for chardakkov. Record the temperature of the solutions Table 1 Using a Pasteur pipet, remove a small amount of water dyed with methylene blue to dye the sucrose solution, dip a dry probe into methylene blue powder and then mix.
The answer will be in Jm -3 which are equivalent to pressure Pa. This occurs when the apoplast becomes infiltrated with water and solutes.
Weigh two or preferably three cores, record your data in Table 2 and then place the cores in one of the test solutions. A “kink” may be observed methoc the graph below the x-axis.
Periodically swirl the containers. What is the water potential of potato tissue?
In this method, tissue samples are weighed before and after incubation in a series of solutions of known osmotic water potential. No change in density, concentration No methof water movement Tissue and solution were at the same WP If we can find this solution concentration —We can find the tissue WP.
This method depends on the change in density in a solution that occurs after a tissue has been immersed in it. This increases the tissue weight and may lead to small errors. Work quickly to minimize evaporation and keep the tissue wrapped in a moist towel.
Record your data in Table 2. Cut them to the same length ca.
Which method do you think will be more accurate? From the graph, determine the concentration of the sucrose solution in which there was no net weight gain i. To make this website work, we log user data and share it with processors.
Complete Tables 1 and 2. OK Any substance that can change shape or flow easily.