Category, Microbiology Laboratory. Test, Test Method Description. Antibacterial Activity Assessment of Textile Materials: Parallel Streak Method. TEST METHODS ARE DELIVERED AS A LINK IN YOUR EMAILED RECEIPT. AATCC TM, Antibacterial Activity Assessment of Textile Materials. Current individual AATCC test methods and other standards for textiles are Fabrics, Assessment of Textile Materials: Parallel Streak Method, Test Method

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AATCC Test Method 147

This may be accomplished more easily by pressing the specimen to the agar surface with a biological section lifter or with a spatula tst has been sterilized by flaming and then air cooled immediately before use. The tset for passing the test must be agreed upon by the interested parties.

Purpose and Scope cluding corresponding untreated controls of the same material, are placed in intimate contact with nutrient agar see 7. Avoid contamination by using good sterile technique in plating and transferring. This is not necessary if prepared, dehydrated medium is used. Allow methdo to gel firmly before inoculating. Wear protective clothing and respiratory protection that prevents penetration by the bacteria.

Rectangular specimens cut 25 – 50 mm They can be any convenient size. A zone of inhibition occurs as a result of the diffusion of an antimicrobial agent from the specimen. Report of results will include an observation of zones of inhibition and growth under the specimen if present. Therefore, every necessary and 5.


The size of the zone of inhibition and the narrowing of the streaks caused by the presence of the antibacterial aatcf permit an estimate of the residual antibacterial activity after multiple washings.

AATCC Test Method Limitations The method is not suitable for materials which are not migratory and are unable to diffuse thru the bacterial growth medium. Many standard textile finishing chemicals, especially crease resistant and permanent press reagents, will often give strong antibacterial activity even after many washes.

At the conclusion of two weeks, make a fresh transplant from stock culture. Wear safety glasses in all laboratory areas. Users of this test method are expressly advised that determination of the validity of any such patent rights, and the risk of infringement of such rights, are entirely their tst responsibility.

Maintenance of Culture of Test Organisms 8. Other organisms can also be used depending on the intended end-use. Check culture purity by making streak plates periodically and observing for a single species-characteristic type of colonies.

The test is followed in the cases where the aarcc agent is migratory i.

AATCC – Antimicrobial Fabric Test | Situ Biosciences LLC

They may be any convenient size. BoxManassas VA ; tel: Some of the bacteria used in this test are pathogenic; i. Specimens of the test material, including corresponding untreated controls of the same material, are placed in intimate contact with growth agar which has been previously streaked with test organism. AATCC MethodAntibacterial Finishes on Textile Materials, Assessment of, is a quantitative procedure which is adequately sensitive but is cumbersome and time consuming for routine quality control and screening tests.

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This test method is not for resale. Check pH and adjust to 7. If no other bacterial species is specified, Staphylococcus aureus may be used as a representative Gram positive organism. Uses and Limitations 1.

AATCC Test Method

Avoid mutation by strict adherence to monthly stock transfers. Plug and sterilize at kPa 15 psi for 15 minutes. Treated materials should be compared to an untreated corresponding material and a material specimen with known bacteriostatic activity.

All OSHA standards and rules must also be consulted and followed.

Notes and References Your comments will receive careful consideration at a meeting of the responsible technical committee, which you may attend. Parallel Streak Method Developed in by AATCC Committee RA31; reaffirmed, ; editorially revised,; revisedwith title change; editorially revised and reaffirmed If you feel that your comments have not received a fair hearing, you should make your views known to Christopher S. This test method is subject to revision at any time by the responsible technical committee and must be reviewed every five years and if not revised, either reaffirmed or etst.

These safety precautions are for information purposes only. The results of using this procedure have been demonstrated by Committee RA31 to be reproducible by various laboratories working with materials containing residual amounts of antibacterial agents meghod determined by chemical assay after mrthod standard washings.